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KMID : 0525720130180030166
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Journal of Chitin and Chitosan
2013 Volume.18 No. 3 p.166 ~ p.169
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Cellular Radical Scavenging Activity of Chitosan-Catechin Conjugate in Mouse Macrophage Cells
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Cho Young-Sook
Je Jae-Young
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Abstract
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Chitosan-catechin conjugate was prepared to examine cellular radical scavenging activity in mouse macrophage cells. No cytotoxicity was observed in the tested concentrations (50~400 μg/mL) of chitosan-catechin conjugate. Chitosan-catechin conjugate inhibited intracellular reactive oxygen species (ROS) formation determined by a fluorescence probe, 2,7-dichlorofluorescin diacetate (DCFH-DA), and the inhibition activity was 32.82% at 200 μg/mL, whereas unmodified chitosan showed 26.29% at the same concentration, indicating ROS scavenging activity was increased by conjugating catechin onto chitosan. Chitosan-catechin conjugate also inhibited lipid peroxidation by 1.07-fold compared with the control (non-treatment group). Furthermore, chitosancatechin conjugate up-regulated the expression of cellular glutathione level by 2.07-fold compared with non-treatment group.
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KEYWORD
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Chitosan, Catechin, Conjugation, Antioxidant activity
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